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更新時(shí)間:2020-09-29 10:55:24瀏覽次數(shù):777次
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人視網(wǎng)膜周細(xì)胞(HRPC)
背景介紹/context:
Human Retinal Pericyte Cells,These antibody-free human primary cells were initiated by centrifugal elutriation following dispersal of normal retinal tissue with collagenase and dispase without the use of positive selection of antibodies. 人視網(wǎng)膜周細(xì)胞提取于正常視網(wǎng)膜組織,經(jīng)酶消化純化,不使用任何陽(yáng)性抗體篩選,細(xì)胞無(wú)抗性。
動(dòng)物種別/Organism:人
組織來(lái)源 Tissue and Cell Type:視網(wǎng)膜組織
形態(tài)/Morphology:上皮細(xì)胞樣,貼壁生長(zhǎng)
培養(yǎng)基和添加劑/Complete Growth Medium and Culture Conditions:
*培養(yǎng)基:CultureBoost™經(jīng)典細(xì)胞培養(yǎng)基(貨號(hào):4N0-500)
氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
*凍存液:細(xì)胞凍存液Cell Freezing Medium(4Z0-705)
人視網(wǎng)膜周細(xì)胞(HRPC)出庫(kù)檢測(cè)指標(biāo):
Standard Tests | |
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
Miscellaneous Tests | |
TESTS | RESULTS |
Cytoplasmic alpha-Actinin Filaments | > 80% positive by immunofluorescence |
Cytoplasmic Desmin Intermediate Filaments | > 80% positive by immunofluorescence |
Cytoplasmic VWF / Factor VIII | < 0.2% positive by immunofluorescence |
Cytoplasmic uptake of Di-I-Ac-LDL | < 0.2% positive by immunofluorescence |
NG2 (Neural/Glial Antigen 2) | >90% positive by immunofluorescence |
PDGFR-beta (Plaet Derived Growth Factor Receptor beta) | >90% positive by immunofluorescence |
GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
Glutamine Synthetase | < 1% by immunofluorescence |
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