克氏錐蟲快速檢測卡（膠體金法）

克氏錐蟲快速檢測卡（膠體金法）

名稱：克氏錐蟲快速檢測卡（膠體金法）

規(guī)格25T/盒

有效期：24個月1-30度保存

預(yù)期用途

錐蟲快速檢測用于診斷克氏錐蟲感染是一種基于特異性多表位重組抗原的快速免疫層析條帶分析。該快速檢測方法用于定性檢測克氏錐蟲曲霉菌衍生抗原的血清抗體。僅供研究使用。不用于診斷程序。

The rapid detection of Trypanosoma cruzi infection is a rapid immunochromatographic strip analysis based on specific multi epitope recombinant antigens. This rapid detection method is used to qualitatively detect serum antibodies against Aspergillus derived antigens of Trypanosoma cruzi. For research purposes only. Not used for diagnostic programs.

通過顯微鏡、血液培養(yǎng)、異種診斷或PCR直接檢測血液中的寄生蟲具有很高的特異性，可以確認(rèn)感染的存在（4，5）。然而，這些程序在技術(shù)和操作上都要求很高。目前使用的其他方法包括測量針對粗制液的抗體、補(bǔ)體固定、間接血凝和熒光抗體（IFA）。所有這些都缺乏特異性和/或敏感性（6-9）。血清學(xué)測試可以檢測寄生蟲不同發(fā)育階段表達(dá)的抗原特異性抗體，非常適合快速簡便地診斷該疾?。?0-14）。為了改進(jìn)查加斯病的血清學(xué)診斷，我們鑒定并使用了一種來源于不同克魯茲弓形蟲抗原的多表位復(fù)合抗原（15，18）。該抗原由總共9個不同的表位組成（15，16）。測試原理此免疫層析測試旨在定性測定抗克魯茲氏桿菌的血清抗體。此測試可檢測克魯茲氏菌感染者的抗體。該測試基于一種專有的黃金混合物，該混合物含有目標(biāo)金綴合物及其與血清和全血中存在的抗體結(jié)合的能力。一旦結(jié)合，金抗體復(fù)合物將橫向移動，與膜上存在的固定化克魯茲錐蟲衍生蛋白形成復(fù)合物，形成測試線。未綁定的黃金將繼續(xù)向上移動，以綁定形成控制線。當(dāng)觀察到測試線時，測試結(jié)果為陽性（見圖1）。

Direct detection of parasites in the blood through microscopy, blood culture, heterologous diagnosis, or PCR has high specificity and can confirm the presence of infection (4,5). However, these programs require high technical and operational requirements. Other methods currently used include measuring antibodies against crude liquids, complement fixation, indirect hemagglutination, and fluorescent antibodies (IFA). All of these lack specificity and/or sensitivity (6-9). Serological testing can detect antigen specific antibodies expressed at different developmental stages of parasites, making it very suitable for rapid and simple diagnosis of this disease (10-14). To improve the serological diagnosis of Chagas disease, we identified and used a multi epitope composite antigen (15,18) derived from different antigens of Toxoplasma cruzi. This antigen consists of a total of 9 different epitopes (15, 16). Testing principle: This immunochromatographic test aims to qualitatively determine serum antibodies against Clostridium cruzi. This test can detect antibodies in individuals infected with Clostridia. This test is based on a proprietary gold mixture that contains the target gold conjugate and its ability to bind to antibodies present in serum and whole blood. Once combined, the gold antibody complex will move laterally and form a complex with immobilized Trypanosoma cruzi derived proteins present on the membrane, forming a test line. Unbound gold will continue to move upwards to bind and form a control line. When the test line is observed, the test result is positive