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型號
HY-136057
品牌
MCE
廠商性質(zhì)
代理商
所在地
杭州市

規(guī)格

10mg 480元 10000支可售

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CAS號	150651-39-1	產(chǎn)地	國產(chǎn)
規(guī)格	10mg	級別	化工級
證書	ISO系列證書

CAS號

150651-39-1

產(chǎn)地

國產(chǎn)

規(guī)格

10mg

級別

化工級

證書

ISO系列證書

iFSP1

iFSP1 是 FSP1 (AIFM2) 的選擇性有效抑制劑，EC₅₀ 值為 103 nM。iFSP1 可選擇性地誘導(dǎo)過表達(dá) FSP1 的 GPX4 敲除細(xì)胞發(fā)生鐵死亡 (ferroptosis)。iFSP1 可增強(qiáng)多種人ai細(xì)胞系對鐵死亡誘導(dǎo)劑的敏感度，如: (1S,3R)-RSL3 (HY-100218A)。

生物活性

iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC₅₀ of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A)^[1].

IC₅₀ & Target

EC50: 103 nM (FSP1)^[1]

體外研究(In Vitro)

iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis^[1].
iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of FSP1, whereas iFSP1 treatment in the FSP1-knockout background had no additive effect to RSL3-induced ferroptosis^[1].
iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines^[1].
AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)^[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Wild-type and Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA
Concentration:	0.001-1 μM
Incubation Time:	24 hours
Result:	Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function.

分子量：323.35

性狀：Solid

Formula：C₂₀H₁₃N₅

CAS 號：150651-39-1

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

DMSO : 20 mg/mL (61.85 mM; Need ultrasonic)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	3.0926 mL	15.4631 mL	30.9262 mL
5 mM	0.6185 mL	3.0926 mL	6.1852 mL
10 mM	0.3093 mL	1.5463 mL	3.0926 mL

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲存時，請?jiān)?6 個月內(nèi)使用，-20°C 儲存時，請?jiān)?1 個月內(nèi)使用。

In Vivo:

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶