Spermine (Synonyms: 精胺; NSC 268508; Neuridine)
Spermine (NSC 268508) 作為一個直接的自由基清除劑,能夠保護(hù) DNA 免受自由基的攻擊。Spermine 具有抗病毒作用。
生物活性
Spermine (NSC 268508) functions directly as a free radical scabenger to protect DNA from free radical attack. Spermine has antiviral effects.
IC50 & Target
Microbial Metabolite
Human Endogenous Metabolite
體外研究(In Vitro)
Spermine (NSC 268508) at physiologically relevant concentrations inhibits ROS-induced DNA damage, with maximal inhibition observed at 1 to 2 mM. These concentrations are well within the estimates for the physiological concentrations of spermine[1].
分子量:202.34
性狀:<29°C Solid,>29°C Liquid
Formula:C10H26N4
CAS 號:71-44-3
運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.
儲存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性數(shù)據(jù)
H2O : 125 mg/mL (617.77 mM; Need ultrasonic)
濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
---|
1 mM | 4.9422 mL | 24.7109 mL | 49.4218 mL |
5 mM | 0.9884 mL | 4.9422 mL | 9.8844 mL |
10 mM | 0.4942 mL | 2.4711 mL | 4.9422 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 儲存時,請在 6 個月內(nèi)使用,-20°C 儲存時,請在 1 個月內(nèi)使用。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
——為保證實(shí)驗結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實(shí)驗的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑: PBS
Solubility: 100 mg/mL (494.22 mM); Clear solution; Need ultrasonic
參考文獻(xiàn)
[1]. Ha HC, et al. The natural polyamine spermine functions directly as a free radical scavenger. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11140-5. [Content Brief]
[2]. Mounce BC, et al. Polyamines and Their Role in Virus Infection. Microbiol Mol Biol Rev. 2017 Sep 13;81(4). pii: e00029-17. [Content Brief]